作　　者： ; ; ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《中国酿造》 2010年第10期113-117,共5页

摘　　要： 研究了酿酒酵母s．cerevisiae YQ-7发酵的最佳培养基组成；通过单因素试验优化碳源和氮源的添加量，响应面法优化培养基中的碳氮比，得出最佳发酵培养基配方：蔗糖76.31g／L，酵母浸膏33．75g／L，酸水解酪蛋白16．00gm，硫酸铵0．5班，硫酸镁1．OgrL，磷酸二氢钾2．6g／L，甘氨酸2g／L。同时，对补糖策略进行了研究，确定补糖工艺为自发酵16h开始补糖，补糖量70％～80％，每隔12h补糖一次，50h终止发酵，生物量为49．47ga,。 The optimal medium composition for Saccharomyces cerevisiae YQ-7 fermentation was studied. The addition amounts of carbon and nitrogen sources were optimized by single factor test, while the C/N ratio of the medium was optimized by response surface methodology. The results showed that the optimal formula of the fermentation medium were sucrose 76.31 g/L, yeast extract 33.75g/L, acid hydrolyzed casein 16.00g/L, （NH4）2SO4 0.5 g/L, MgSO4 1.0 g/L, KH2PO4 2.6 g/L and glycin 2 g/L. The sugar-feeding strategy was also investigated and determined as follows： the sugar-feeding started at 16 h of self-fermentation with 70%-80% sugar, and repeated at 28 h and 40 h. The biomass reached 49.47 g/L when the fermentation ended at 50 h.

关 键 词： 酿酒酵母 培养基优化 响应面法

领　　域： [轻工技术与工程] [轻工技术与工程]