作　　者： ; ; ; ; ; (张胜凯）;

机构地区： 河北省石家庄市质量技术监督局新华区分局河北石家庄050060

出　　处： 《安徽农业科学》 2010年第22期11727-11729,共3页

摘　　要： [目的]建立快速检测沙门氏菌的环介导等温扩增(LAMP)技术。[方法]以沙门氏菌的特异性基因(invA)序列为靶序列设计引物,并对该序列进行等温扩增;对Mg2+浓度、反应温度和反应时间扩增条件进行优化,并对沙门氏菌进行检测和鉴定。[结果]在Mg2+浓度为2.0mmol/L时,62℃反应40min,扩增可达最佳效果,LAMP法检测的灵敏度为7.8cfu/ml。[结论]建立了快速检测沙门氏菌的LAMP技术,该方法灵敏度高、耗时短、方法简便,便于在基层推广,为检测食源性致病菌构建了一个技术平台。 [Objective] The research aimed to establish loop-mediated isothermal amplification （LAMP） technology for rapid detection of Salmonella.[Method] The primers were designed according to the Salmonella-specific gene （invA） sequence as the target sequence.And the sequence was amplified under the isothermal conditions.The reaction conditions were optimized including Mg2＋ concentration,reaction temperature and time etc.And Salmonella stains were detected and identified.[Result] The results indicated that the best amplification conditions was 62 ℃ for 40 min when Mg2＋ concentration was 2.0 mmol/L.The sensitivity of LAMP detection of Salmonella stains was 7.8 cfu/ml.[Conclusion] The rapid method of LAMP detection of Salmonella was established.LAMP method was very rapid,sensitive and convenient,suitable for grass-roots generalization.This method built a technology platform for the detection of food-borne pathogens.

关 键 词： 环介导等温扩增 沙门氏菌 检测

领　　域： [生物学]