作　　者： ; ; ; ;

机构地区： 华南理工大学轻工与食品学院制浆造纸工程国家重点实验室

出　　处： 《华南理工大学学报（自然科学版）》 2010年第8期34-39,共6页

摘　　要： 以卫生纸厂初级污泥作为生物质原料,采用同步糖化发酵法生产燃料乙醇,对其工艺条件进行了优化,并考察了3种非离子表面活性剂(Tween-20、Tween-60、Tween-80)对造纸污泥同步糖化发酵的促进效果.结果表明:合适的工艺条件为反应温度36℃、底物质量浓度100 g/L、酵母接种量6%(体积分数)、纤维素酶用量25 FPU/g;Tween-20能显著促进造纸污泥的同步糖化发酵,添加0.2%的Tween-20时,乙醇产率提高14%;上述工艺条件下反应48 h的乙醇质量浓度达19.5 g/L,是理论值的63.9%.对同步糖化发酵前后污泥及发酵液的主要化学成分进行分析,发现大量纤维素已糖化发酵成乙醇,仅少量半纤维素发生水解,水解产物木糖不能被酵母有效利用发酵成乙醇.扫描电镜分析显示造纸污泥经同步糖化发酵后,长条状的纤维大多被破坏,同时出现了大量椭球状的酵母细胞. Primary sludge from a toilet paper mill was used as biomass material to produce fuel ethanol via simulta- neous saeeharifieation and fermentation （SSF）, and three different non-ionic surfaetants, i.e. , Tween-20, Tween-60 and Tween-80, were evaluated for their ability to enhance SSF. The SSF process conditions were then optimized at a temperature of 36 ℃, with a substrate mass concentration of 100 g/L, an inoculums size of 6% （volume fraction）, and a cellulase dosage of 25 FPU/g. The results show that Tween-20 significantly accelerates the ethanol production. Specifically, the ethanol yield increases by 14% with a Tween-20 dosage of 0.2%. In the optimal conditions, the mass concentration of ethanol reaches 19.5 g/L after a 48 h incubation, which is 63.9% of the theoretical one. An analysis of the main chemical compositions of the paper sludge before and after SSF and of the fermentation liquor shows that a large amount of cellulose is converted into ethanol and only a small amount of hemicellulose is hydrolyzed, and that the hydrolysate xylose cannot be effectively used by yeast for ethanol production. Moreover, SEM results indicate that, after SSF, the long linear fibers in the paper sludge are destroyed and a lot of ellipsoidal yeast cells appear simultaneously.

关 键 词： 造纸污泥 纤维素酶 酿酒酵母 乙醇 表面活性剂

领　　域： [轻工技术与工程] [化学工程]