作　　者： ; ; ; ;

机构地区： 广东海洋大学农学院

出　　处： 《农业生物技术学报》 2010年第4期801-806,共6页

摘　　要： 红树(Acanthus ilicifolius)内生细菌AiL3菌株通过形态学观察、生理生化特征测定及16S rDNA序列分析鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens)。菌株培养滤液经有机溶剂萃取和硫酸铵沉淀分析发现,硫酸铵沉淀物具有抑菌活性,说明AiL3菌株产生的抗菌活性物质可能是蛋白类物质。该蛋白粗提液对芒果炭疽病菌(Colletotrichum gloeosporioides)、黄瓜枯萎病菌(Fusarium oxysporum f.sp.cucumerinum)等8种植物病原菌均有较强的拮抗作用;对芒果炭疽病菌菌丝生长和分生孢子萌发的EC50分别为9.32和5.81μg/mL,当提取物浓度为20.33μg/mL时,可导致芒果炭疽病菌分子孢子的消解。 Based on the morphological,physiological and biochemical characteristics and 16S rDNA sequences analysis,strain AiL3 isolated from mangrove （Acanthus ilicifolius） was identified as Bacillus amyloliquefaciens.Antifungal substance from fermentation of strain AiL3 was deposited by the method of organic solvent extraction and ammonium sulfate,exhibited a high antifungal activity of precipitated by 50% ammonium sulphate precipitation.The results implied that antifungal substance of strains AiL3 was a kind of proteins.It was shown on PDA plates that the development of many pathogens such as Colletotrichum gloeosporioides and Fusarium oxysporum f.sp.cucumerinum was inhibited by the antifungal protein effectively.Their EC50 values were 9.32 and 5.81μg/mL on mycelial growth and conidium germination of C.gloeosporioides.Furthermore,the antagonistic crude protein（20.33μg/mL） could degrade the conidial cell wall and restrain completely the formation of spore.

关 键 词： 红树 内生细菌 鉴定 抗菌活性物质

领　　域： [农业科学] [农业科学]