作　　者： ; ; ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《应用与环境生物学报》 2010年第4期534-540,共7页

摘　　要： 采用溶液培养法研究培养基的碳源、氮源和无机磷浓度对南美蟛蜞菊(Wedelia trilobata)毛状根生长以及其提取物对水稻、鬼针草、马唐种子萌发的影响.结果表明,当培养基含糖量小于5%时,以葡萄糖为碳源促进南美蟛蜞菊毛状根生长的效果最好,蔗糖次之;而大于5%时仅麦芽糖仍能促进其生长,而当麦芽糖浓度大于7%时,其生长也逐渐被抑制.培养基中添加(NO_3)^--N(263.1μg/mL)和(NH_4)^+-N(577.2μg/mL)组合时,或无机磷含量为MS培养基无机磷用量的2～3倍时对毛状根生长的促进效果最佳;培养基缺Ca2+时,毛状根变得纤细,分枝多,生长旺盛;但随着培养基Ca^(2+)浓度的增加,其生长受到抑制.毛状根的乙醇提取液比水提取液对水稻(Oryza sativaL.)、鬼针草(Bidens pilosaL.)、马唐(Common crabgrass herb)种子的萌发抑制作用强,其水提取液处理的水稻、鬼针草、马唐种子的萌发率分别是84%、93%、20%,而乙醇提取液处理的马唐种子萌发率只有3%,水稻和鬼针草种子则完全不萌发.与野生根的乙醇提取液相比,毛状根的乙醇提取液对水稻、鬼针草、马唐种子的萌发抑制作用更强.暗培养毛状根的乙醇提取液对水稻、鬼针草、马唐种子萌发的抑制作用要比光照培养的毛状根乙醇提取液的抑制作用强,光照培养的毛状根乙醇提取液处理的水稻、鬼针草、马唐种子的萌发率分别是28.7%、3.9%、18.7%,暗培养毛状根的乙醇提取液处理的水稻、马唐种子萌发率只有4%和1%,鬼针草则完全不萌发.本实验为今后规模培养南美蟛蜞菊毛状根选择合适的培养条件及将南美蟛蜞菊毛状根中的次生代谢产物应用于农业生产奠定了实验与技术基础. The effects of carbon and nitrogen sources and inorganic phosphorus concentrations on the growth of Wedelia trilobata hairy roots were investigated, as well as the effect of their extract on seed germination. The results showed that when cultured with sugar content less than 5%, the best growth of W. trilobata hairy roots was obtained with glucose as carbon source, followed by sucrose, while with the sugar content more than 5%, only maltose was able to promote the hairy root growth. However, when maltose concentration was higher than 7%, the growth of hairy roots was also gradually inhibited. The best stimulating effect on the growth was obtained when （NO_3）^--N （263.1 μg/mL） was combined with （NH_4）~＋-N （577.2 μg/mL） or supplemented with inorganic phosphorus content 2~3 times the amount of inorganic phosphorus in MS medium. When cultured without addition of Ca^（2＋） into the medium, the hairy roots had vigorus growth with many branches and became thinner, but with the increasing concentration of Ca^（2＋）, their growth was suppressed. The inhibitory effect of seed germination was higher when the seeds of rice （Oryza sativa L.）, Bidens pilosa L., and crabgrass were soaked with ethanol-soluble extract, than that of those treated with water-soluble extract. Compared to the control, when soaked with water-soluble extract of hairy roots, the seed germination rates of rice, B. pilosa and crabgrass were 84%, 93% and 20%, respectively, but when soaked with ethanol-soluble extract of hairy roots, the seed germination rate of crabgrass seeds was 3%, but no seed germination of rice and B. pilosa was observed The seed germination was more inhibitory when with ethanol-soluble extract of hairy roots than that of wild roots. When the seeds of rice, B. pilosa and crabgrass were soaked with ethanol-soluble extract of light-cultured hairy roots, their seed germination rates were 28.7%, 3.9% and 18.7%, respectively, and were higher than those with ethanol- soluble extract of dark-cultured hairy roo

关 键 词： 南美蟛蜞菊 毛状根 浸提液 它感作用 种子萌发

领　　域： [生物学] [生物学]