作　　者： ; ; ;

机构地区： 广东海洋大学食品科技学院

出　　处： 《食品科技》 2010年第8期49-54,共6页

摘　　要： 以军曹鱼头为原料,ACE抑制率为指标,对木瓜蛋白酶水解鱼头蛋白的工艺条件进行优化,筛选具有较高抑制ACE活性的生物功能活性肽。结果表明:优化水解工艺条件为温度73℃、初始pH值6.0、加酶量2300U/g,料液比1∶2,水解时间4h,水解产物对ACE抑制率达到最大值为82.63%,此时水解度为17.21%。 The technology for preparation of angiotensin-I-converting enzyme inhibitory peptides by hydrolyzing heads of cobia with papain was optimized by using the index of the Angiotensin-I-Converting Enzyme（ACE） inhibitory activity.The peptides with high ACE inhibitory activities were selected.Experimental results indicated that the peptides with optimum ACE inhibitory activity were obtained when the enzymic hydrolysis parameters are composed of 73℃,pH 6.0 and enzyme dosage 2300 U/g,ratio of matieral to water was one to two,hydrolysis time was four hours.In those conditions,the ACE inhibitory activity was the maximum with 82.63% and the degree of hydrolysis was 17.21%.

关 键 词： 军曹鱼鱼头 酶解 抑制肽 响应面

领　　域： [轻工技术与工程] [轻工技术与工程]