作　　者： ; ; ; ; ; ; ; ; (史卫军）;

机构地区： 深圳出入境检验检疫局

出　　处： 《中国兽医科学》 2010年第8期793-796,共4页

摘　　要： 利用pFastBacHTA质粒将非洲马瘟病毒(AHSV)血清4型的VP7基因重组入杆状病毒,并感染昆虫细胞Sf9。结果显示,VP7蛋白在真核细胞内获得了表达,但表达量较低。免疫印迹试验证实,VP7重组蛋白具有较好的免疫原性,将其作为检测抗原包被酶标板,初步建立了检测AHSV的间接酶联免疫吸附试验方法。 In order to obtain the VP7 protein,which has the same or similar antigenicity as that of the virus,the plasmid of pFastBac HT A with AHSV-4 serotype VP7 gene was recombined into the baculovi-rus,and infected insect cells Sf9.The VP7 protein was expressed within eukaryotic cells,but the expression level was low.Western-blotting and indirect ELISA showed that the expressed VP7 protein had good immunogenicity,and could be used as antigen for clinical detection of African horse sickness.Using the expressed VP7 protein,an indirect ELISA was developed for detection of African horse sickness virus.

关 键 词： 非洲马瘟病毒 蛋白 真核表达 抗原性

领　　域： [农业科学] [农业科学] [农业科学]