作　　者： ; ;

机构地区： 广州中医药大学中药学院

出　　处： 《遗传》 2010年第7期719-725,共7页

摘　　要： 参照近缘物种线粒体基因序列共设计和合成了8对引物,结合Ex Taq-PCR、TA克隆和步移测序技术,文章首次获得眼镜王蛇线粒体基因组全序列(GenBank登录号:EU_921899)。该基因组全长17 267 bp,共编码13个蛋白、2个rRNA、23个tRNA——其中tRNA-Ile基因发生了复制,属于一种新的蛇类物种线粒体基因排列模式,另外还含有2个非编码的富含AT的控制区。除了8个tRNA基因和1个蛋白基因由L链编码外,其余均由H链编码,其中H链编码基因的A和T含量接近,而L链上A的含量则明显高于T。基于21种蛇合并的"12S+16S"rRNA基因序列的系统发育分析表明,眼镜王蛇属与眼镜蛇属亲缘关系较近,两者与环蛇属共同构成一个单系群。作为国内外眼镜王蛇线粒体基因组全序列的首次报道,上述结果对于蛇类物种分子系统发育和进化研究具有重要意义。 We obtained the complete mitochondrial genome of King Cobra（GenBank accession number： EU_921899） by Ex Taq-PCR, TA-cloning and primer-walking methods. This genome is very similar to other vertebrate, which is 17 267 bp in length and encodes 38 genes （including 13 protein-coding, 2 ribosomal RNA and 23 transfer RNA genes） and two long non-coding regions. The duplication of tRNA-lle gene forms a new mitochondrial gene rearrangement model. Eight tRNA genes and one protein genes were transcribed from L strand, and the other genes were transcribed genes from H strand. Genes on the H strand show a fairly similar content of Adenosine and Thymine respectively, whereas those on the L strand have higher proportion of A than T. Combined rDNA sequence data （12S＋16S rRNA） were used to reconstruct the phylogeny of 21 snake species for which complete mitochondrial genome sequences were available in the public databases. This large data set and an appropriate range of outgroup taxa demonstrated that Elapidae is more closely related to colubridae than viperidae, which supports the traditional viewpoints.

关 键 词： 眼镜王蛇 线粒体基因组 系统发育

领　　域： [生物学]