作　　者： ; ; ; ; ;

机构地区： 广西科技大学生物与化学工程学院

出　　处： 《化学与生物工程》 2010年第7期87-91,共5页

摘　　要： 首次建立了以金电极为工作电极电致化学发光测定盐酸苯海索的方法,采用循环伏安法和电致化学发光法研究了盐酸苯海索-Ru(bpy)32+体系的电化学行为和电化学发光行为。结果表明,在pH值8.0的磷酸盐缓冲溶液中,扫描速率为0.10 V.s-1时,电致化学发光(ECL)强度值与盐酸苯海索浓度在2.00×10-7~1.00×10-4mol.L-1范围内呈良好线性关系,其线性回归方程为:I(Counts)=300.00×105c-120.71,方法检出限为1.00×10-8mol.L-1(s/n=3)。不同时段测定5.9184×10-5mol.L-1的盐酸苯海索片溶液4次,所测样品含量值的RSD为1.55%。对样品进行加标回收率实验,回收率为97.50%~104.00%。该方法具有较好的稳定性和灵敏度,样品处理简单快速,用于盐酸苯海索片的测定,效果良好。 Electrochemiluminescence（ECL）intensity of Ru（bpy）3^2＋ could be enhanced in the presence of benzhexol hydrochloride in basic solution.Based on this,a novel ECL method was established for the determination of benzhexol hydrochloride at gold electrode.The electrochemical and ECL characteristics of Ru（bpy）3^2＋-benzhexol hydrochloride system were investigated by cyclic voltammetric（CV） and ECL method,and the experimental conditions were optimized.Under the optimal conditions,the enhanced ECL intensity had good linear relationship with the concentration of benzhexol hydrochloride in the range of 2.00×10^-7～1.00×10^-4 mol·L^-1,respectively,with a detection limit of 1.00×10^-8 mol·L^-1（s/n=3）.The RSD for 4 times determination of 5.9184×10^-5 mol·L^-1 benzhexol hydrochloride was 1.55% and the spiked recoveries were 97.50%～104.00%.The method showed high stability and sensitivity and was applied to the determination of benzhexol hydrochloride in tablet with satisfactory results.

关 键 词： 金电极 电致化学发光 盐酸苯海索

领　　域： [理学] [理学]