作　　者： ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《上海海洋大学学报》 2010年第4期482-488,共7页

摘　　要： 2008年5月,湛江市某网箱养殖场的卵形鲳鲹(Trachinotus ovatus)幼鱼发生大规模死亡,调查发现病鱼呈现体色发黑、反应迟钝、呈螺旋状或旋转游动等典型的病毒性神经坏死症症状,在鱼体没有发现寄生虫或细菌感染,PCR检测发现病鱼感染了鱼类神经坏死病毒(NNV)。利用已经发表的NNV核酸序列设计引物,克隆外壳蛋白基因并测序,根据同源性比较和系统进化分析,该病毒与斜带石斑神经坏死病毒(ECNNV)碱基相似率达99.2%,属于赤点石斑鱼神经坏死病毒基因型(RGNNV)。同时进行人工感染试验,采用4种方法感染该病毒,累计死亡率均达100%,并对感染样品进行克隆测序鉴定,证明导致此次湛江卵形鲳鲹大规模死亡的病原为神经坏死病毒。 Investigation on mass mortality of Trachinotus ovatus juveniles cultured in sea cage in Zhanjiang was carried out.The symptom of the sick fish was as follows：the skin was nigrescent,it swam twisty or circularly,responded slowly and anorexia.There was no infection of parasites or bacteria. The fish nervous necrosis virus was observed in the fish by PCR analysis. Two pairs of primers were designed based on the homogenus gene sequences from other fish nervous necrosis virus （NNV） submitted to GeneBank and the coat protein gene of Trachinotus ovatus nervous necrosis virus （PNNV） was cloned and sequenced. According to the analysis of similarity and phylogenelysis,the coat protein gene from PNNV had the highest identity of 99.2% to Epinephelus coioides nervous necrosis virus （ECNNV） at nucleotide level. Based on the result of molecular phylogenetic analysis,PNNV belonges to red-spotted grouper nervous necrosis virus （RGNNV） genotype. The cumulative mortality of all groups infected were 100% in artificial infection experiment by four kinds of infection. The infected samples were cloned and sequenced.These results suggested that the pathogen of mass mortality of Trachinotus ovatus juveniles cultured in sea cage in Zhanjiang was NNV.

关 键 词： 卵形鲳鲹 神经坏死病毒 序列分析

领　　域： [农业科学] [农业科学]