作　　者： ; ; ; ;

机构地区： 昆明理工大学环境科学与工程学院

出　　处： 《农业环境科学学报》 2010年第7期1254-1260,共7页

摘　　要： 分别采用水培和土培实验方法,研究了两种培养方式下砷对砷超富集植物大叶井口边草(Pteris cretica var.nervosa)生理生化的影响。将大叶井口边草分两组培养:一组置于0、5、20、50 mgAs.L-1含砷Hoagland营养液下,另一组置于0、50、100和200 mgAs.kg-1土培条件下。培养14 d后,测定叶片光合色素、游离脯氨酸、可溶性糖含量、过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性等指标。结果表明,砷胁迫导致大叶井口边草叶片叶绿素、游离脯氨酸和可溶性糖含量以及POD、CAT、SOD活性发生明显变化。随着砷浓度增加,无论水培和土培方式,相对于对照,叶绿素a、a+b含量均显著减少(P<0.05),但叶绿素a/b无显著差异(P>0.05),游离脯氨酸和可溶性糖含量显著增加(P<0.05),CAT活性显著降低(P<0.05)。游离脯氨酸和可溶性糖对砷胁迫下大叶井口边草的砷耐性起了重要作用。 Effects of arsenic（As）on physiological and biochemical properties of Pteris cretica var.nervosa,an As hyperaccumulator,were e-valuated under two culture conditions（hydroponic and pot trials）.Seedlings of Pteris cretica var.nervosa were divided into two groups.One group was exposed to Hoagland nutrition solution containing 0,5,20 and 50 mgAs.L-1.The other group was exposed to soil spiked with 0,50,100 and 200 mgAs.kg-1.After 14 d,concentrations of photosynthetic pigments,free proline,soluble sugar and activities of peroxidase（POD）,catalase（CAT）and superoxide dismulase（SOD）in plant fronds were determined.Significant changes of the parameters mentioned above were observed under As treatments.Increased As concentrations under both culture conditions resulted in a significant decrease in chloro-phyll a,a＋b contents and CAT activity,an insignificant change in chlorophyll a/b,and a significant increase in free proline and soluble sugar contents.It was concluded that free proline and soluble sugar might play an important role in As tolerance of Pteris cretica var.nervosa.

关 键 词： 培养方式 砷 大叶井口边草 生理生化

领　　域： [环境科学与工程]