作　　者： ; ; ; ; ; ;

机构地区： 南京医科大学基础医学院生物化学与分子生物学系

出　　处： 《现代生物医学进展》 2010年第11期2057-2060,共4页

摘　　要： 目的:表达人卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)N端第18-34氨基酸片段。方法:将FSHRN端第18-34氨基酸片段克隆至pGEX4T-1中,构建成重组质粒pGEX4T-1-FSHRN(18-34位氨基酸)。将该重组质粒转化E.coliBL21后,IPTG诱导其表达,经亲和层析进行分离纯化,western blot鉴定。结果:克隆成功,并表达FSHRN端第18-34氨基酸片段,所表达的融合蛋白中60%为可溶性蛋白。结论:成功克隆、表达、纯化了人FSHRN端第18-34氨基酸片段,为后续动物免疫试验提供抗原。 Objective：To produce the N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor.Methods：The N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor was cloned and translated into pGEX4T-1 vector.The expression plasmid was translated into E.coli strain BL-21.The GST fusion protein was induced by IPTG and purified to homogeneity by affinity chro-matography;Then protein was identified by western blot.Results：The recombinant GST-FSHR N fusion protein was expressed and purified successfully, of which 60% was soluble.Conclusion：The expression and purification of N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor would provide antigen for the further animal experiment.

关 键 词： 表达 纯化

领　　域： [生物学]