作　　者： ; ; ;

机构地区： 华南理工大学轻工与食品学院制浆造纸工程国家重点实验室

出　　处： 《应用基础与工程科学学报》 2010年第3期472-483,共12页

摘　　要： 针对厌氧处理过程中造纸法烟草薄片废水中的烟碱生物降解率不高的问题,提出以反硝化反应强化烟碱厌氧生物降解的思路.以UASB小试反应器中的厌氧颗粒污泥为研究对象,通过外加硝酸盐来诱导驯化厌氧颗粒污泥中反硝化菌的代谢活动,并利用PCR-DGGE分子生物技术分别对接种污泥、反硝化驯化前后的厌氧颗粒污泥微生物群落结构进行了分析.研究结果表明经过反硝化驯化,厌氧反应器出水中烟碱的浓度可由120mg/l以上降至25mg/l左右,CODCr由600mg/l左右降至200—300mg/l,反硝化驯化有效富集了厌氧颗粒污泥中与反硝化反应和烟碱降解相关的菌属,实现了造纸法烟草薄片废水中烟碱的有效降解和废水CODCr的深度去除. In order to solve the problem that nicotine in paper-making tobacco sheets wastewater was not biodegraded much in anaerobic treatment process,denitrification as a bio-augmentation technique was induced into the domestication of anaerobic granular sludge in UASB batch bioreactor,nitrate was added into the wastewater to induce the metabolism activity of anaerobic denitrifying bacterium,the microbial flora and microbial diversity in different sludge were analyzed by molecular biology technology PCR-DGGE.The results showed that through domestication,the nicotine concentration in the effluent water of anaerobic reactor was reduced from above 120mg/l to about 25mg/l and the concentration of CODCr decreases from about 600mg/l to 200—300mg/l,and the enrichment of denitrifying bacterium in nitrate conditions resulted in the advanced biodegradation of nicotine and CODCr in wastewater.

关 键 词： 造纸法烟草薄片 厌氧颗粒污泥 烟碱 反硝化

领　　域： [环境科学与工程]