作　　者： ; ; ;

机构地区： 暨南大学生命科学技术学院生物医学工程系

出　　处： 《高分子通报》 2010年第7期50-56,共7页

摘　　要： 材料表面固定生物分子一直都被认为是其提高其细胞相容性有效的途径。本文采用羧基二咪唑(CDI)作为缩合剂,使p(HEMA-MMA)表面羟基与胶原中氨基发生缩合反应,从而将I-型胶原接枝在材料表面。然后利用"接枝-吸附"的方法,将I-型胶原和bFGF混合层吸附固定于材料表面,形成稳定的胶原与bFGF涂层。红外光谱(FTIR)和原子力显微镜(AFM)证实了该种固定方法的成功。静态接触角显示材料表面亲水性较改性前略微降低。涂层稳定性实验结果表明在类似人体环境(pH=7.4)下涂层具有良好的稳定性。细胞粘附性实验以及MTT实验一致显示改性后材料的细胞相容性得到明显提高。因此,Col/bFGF-p(HEMA-MMA)有望成为能够被宿主机体所接受的生物材料。 Immobilization of bimolecular onto the polymer surface represents an effective approach to improve its cytocompatibility. In this paper,CDI was used as a condensing agent to catalyze the reaction between carboxyl groups and amino groups. As a result,type-I collagen was covalently immobilized onto p（HEMA-MMA） hydrogel surface. Then the mixed-layer of type-I collagen and basic fibroblast growth factor （bFGF） were coated onto p（HEMA-MMA） hydrogel surface by the method of grafting and coating,finally a stable biocompatible layer was formed. The immobilization was proved successfully by the result of AFM and FTIR analysis. The result of contact angles testing showed that the hydrophilicity of modified material decreased slightly. The layer was rather stable in body-like environment （pH=7.4）. The results of cell adhesion assay and MTT assay conformably demonstrated that the cytocompatibility of modified material was improved significantly. Therefore,the collagen and bFGF immobilized p（HEMA-co-MMA） hydrogel hold great promise to be a biocompatible material.

关 键 词： 型胶原 碱性成纤维生长因子 接枝 吸附

领　　域： [理学] [理学]