作　　者： ; ; ; ;

机构地区： 华南农业大学

出　　处： 《华中农业大学学报》 2010年第3期282-286,共5页

摘　　要： 在室内条件下,测定了红火蚁Solenopsis invicta Buren工蚁感染球孢白僵菌Beauveria bassiana后体内保护酶和酯酶的活性。结果表明:红火蚁工蚁被球孢白僵菌感染后,体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性都发生了不同程度的改变,SOD和CAT的活性除在接种后12 h与对照相比无显著差异外,其他处理与对照相比均存在显著差异,且各处理酶的活性显著低于对照;而POD的活性则是先上升后下降,在接种后的36 h其活性为12.33 U3,显著高于对照(10.13 U3),随后呈下降趋势;羧酸酯酶(CarE)的活力呈先下降后上升的趋势,在接种后36 h活性最小(6.55 U4),与对照相比差异显著(8.15 U4),之后不断上升;乙酰胆碱酯酶(AchE)比活力则呈先上升后下降的趋势,在接种后36 h时最高(9.24 U5),显著高于对照(6.65 U5),之后不断下降。 Solenopsis invicta Buren（red imported fire ant,RIFA） was one of the most dangerous and devastating invasive pests in the world.In this paper,the changes of the activities of superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD）,carboxylesterase（CarE） and acetylcholinestrease（AchE） in red imported fire ant workers infected by Beauveria bassiana were studied,which provided the theoretical basis for the research on red imported fire ant infected by Beauveria bassiana and the pathogenic mechanism.The results showed that the activities of SOD,CAT and POD in infected red imported fire ant workers＇ bodies had changed in different degrees.12 hours after the inoculation,the activities of SOD and CAT of infected red imported fire ant workers revealed significant difference in contrast to the untreated ones,and their enzyme activities were significantly lower than those of the controls,their POD activity was first went up and then went down;36 hours after the inoculation,the activity of 12.33 U3 was significantly higher than that of the control group,which was 10.13 U3,but then it went down.The activity of CarE in 36 hours after inoculation was the lowest,which was 6.55 U4,greatly different from the 8.15 U4 of the control group.The activity of AchE went up at first and then went down,and reached the highest point at 9.24 U5 36 hours after the inoculation,which was significantly higher than the 6.65 U5 of the controls.

关 键 词： 红火蚁 球孢白僵菌 保护酶 酯酶

领　　域： [农业科学] [农业科学]