作　　者： ; ; ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《水产学报》 2010年第6期656-664,共9页

摘　　要： 运用通用引物长距PCR(Long-PCR)和常规PCR相结合的方法测定了鲈形目笛鲷科的4种笛鲷属鱼类(孟加拉笛鲷、四带笛鲷、千年笛鲷和马拉巴笛鲷)和军曹鱼科的军曹鱼线粒体DNA基因组全序列(GenBank序列号分别为FJ171339,FJ416614,FJ824741,FJ824742和NC_011219),得出所用全序列测定体系的方法通用性较强,操作简单。线粒体基因组的比对分析表明,测定的mtDNA基因组的绝大部分区段与GenBank中现有的脊椎动物的序列有较高的同源性。以军曹鱼外群结合GenBank中近缘笛鲷鱼类(勒氏笛鲷、蓝点笛鲷和黑带鳞鳍梅鲷)进行的聚类分析中,勒氏笛鲷与黑带鳞鳍梅鲷的聚类关系近于同属物种,与形态学分类存在矛盾。通过单个基因和基因拼接序列比较,综合考虑邻位连接法构建系统进化树的置信度和序列的信息量,对13种蛋白质编码基因在属内种间的系统进化分析能力进行了评估,将基因分成不同的4等:很好的为ATPase6和cox2;好的序列为ND2和cox1;差的为ND6、ND3和ATPase8;包括Cytb在内的其余6种基因为中等。分析还揭示出序列长度的增加可以提高系统进化树的置信度,且属内物种间比较时序列长度的影响小于高级阶元。 The entire mitochondrial DNA sequence （mitogenome）of five species, Lutjanus bengalensis, Lutjanus malabaricus, Lutjanus sebae, Lutjanus kasmira and cobia（ Rachycentridae ： Rachycentron canadum ） were determined using long PCR and primer-walking methodology with universal primers, and their GenBank Accession numbers are FJ171339, FJ416614, FJ824741, FJ824742 and NC _011219, respectively. The mitochondrial genomic sequence in snappers and cobia are similar to other vertebrate mitochondrial genomes with respect to gene order and genomic organization. Phylogenetic relationships derived from mtDNA sequences of Lutjanus russellii, Lutjanus rivulatus, Lutjanus bengalensis, L. malabaricus, L. sebae, L. kasmira, cobia and dark banded fusilier （Caesioninae：Pterocaesio tile）were incongruent with those derived from morphological taxonomic analyses. Beyond expected, high homogeneities were observed between some lutjanids and fusilier, belonging to Lutjaninae and Caesioninae. Further efforts may clarify the phylogenetic relationships among these snappers. Moreover, we investigated the performance of all thirteen mitochondrial protein-coding genes to recover the phylogenies. According to these tests, mitochondrial protein-coding genes can be roughly classified into four groups at the level of genus：of very good （ATPase 6 and cox2 ）, good （ coxl and ND2 ） ,medium（ cox3 ,NDI,ND4 ,ND4L and ND5 ）, and poor（ ND3 ,ND6, and ATPase 8 ）. And we inform that simple length differences and rate differences between these genes cannot account for their different phylogenetic performance. We advice the careful selected genes and their combinations should be helpful for high resolved phylogenetic analysis for different level taxon.

关 键 词： 笛鲷属 线粒体基因组 分子系统进化

领　　域： [农业科学]