机构地区: 中山大学生命科学学院
出 处: 《海洋科学》 1999年第1期48-50,共3页
摘 要: 采用PCR及序列测定的方法,对在厦门西港海域采集的赤潮铜绿微囊藻16S和 235rDNA基因间隔区Intergenic Spacer Region (ISR)区进行了序列测定和分析,并通过与两 种淡水微囊藻的比较,找出了其特征性核苷酸作为专一性分子探针设计的靶序列,为该藻 种以及微囊藻属的快速鉴定及系统学研究提供了分子基础。 The rDNA Intergenic Spacer Region (ISR) was sequenced and analyzed from a 'Red Tide' species Mlerocystis aeruginoea collected from Xiamen. The spacer sequences was 360 bp in lengh exclusive of 16S and 23S rDNA coding regions,containing a lle tRNA gene. Based on the analyses of the obtained sequences and other two sequences from fresh water strains, the evolutionary affiliation between species was discussed.