作　　者： ; ;

机构地区： 中山大学生命科学学院水生经济动物研究所

出　　处： 《水产学报》 1999年第1期1-5,共5页

摘　　要： 根据大鳍脑垂体匀浆和血清样品的稀释曲线与鲤生长激素（ｃＧＨ）标准曲线的平行性，采用鲤生长激素的标准品和抗血清（ＲＡＧ）测定了周年中几个不同时期大鳍脑垂体和血清样品的生长激素（ＧＨ）含量，发现脑垂体和血清中的ＧＨ含量均表现出明显的季节变化。脑垂体的ＧＨ含量分别在３月份和８月份出现两个峰。４～７月的繁殖期和１１～１月的越冬期间，脑垂体的ＧＨ含量很低，而且波动不大。受水温和光周期的影响，大鳍血清ＧＨ水平表现为从冬季（１１～１月）到春季（２～４月）逐渐上升，夏季急剧升高，到夏末（７月底）达到最高，一直持续到秋季。大鳍血清ＧＨ含量的变化与生殖周期密切相关，最低的ＧＨ含量出现在性腺静止期，其次为性腺发育期，再次为性腺成熟期，在产卵期急剧升高，最高为性腺退化期。 Doseresponse inhibition curves for serial dilutions of sera and pituitary homogenates from the bagrid catfish M. macropterus (Bleeker) parallel that for the common carp growth hormone (cGH), so the radioimmunoassay for cGH was used to assay the blood and pituitary GH levels of the catfish. Seasonal changes were found in both pituitary GH contents and serum GH levels. Seasonal changes in pituitary GH contents were marked by two distinct peaks, one was in March and the other was in August, just anterior to and posterior to the gonadotropin (GTH) peak(April to July) of the pituitary. From April to July, the reproductive period of the fish, and from November to February, the overwinter period, the pituitary GH contents were low and with minor variations. Influenced by water temperature and photoperiod, the lowest level of serum GH was observed in winter and a progressive increase was found in spring and summer. But a significant increase was found at the end of summer (July), and remained a high level in autumn. Seasonal changes in serum GH levels were also correlated to reproductive cycle. The lowest serum GH level was observed in the resting period, elevated gradually with the recrudescence of the gonads, increased significantly at the spawning period, and got the highest value in the sexual regressed period. These results were in accordance with those obtained in common carp and goldfish.

关 键 词： 大鳍 脑垂体 血清 生长激素 季节变化

领　　域： [生物学]