作　　者： ; ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院生物工程研究所

出　　处： 《中国病理生理杂志》 2010年第5期931-936,共6页

摘　　要： 目的:探讨重组人骨形成蛋白-2(rhBMP2)对人乳腺癌细胞MCF-7迁移性的影响。方法:用rhBMP2诱导人乳腺癌细胞MCF-7,并利用原子力显微镜观察细胞的形态结构变化。通过划痕愈合和transwell实验研究rhBMP2诱导下人乳腺癌细胞MCF-7迁移能力的变化。结果:rhBMP2诱导组的细胞形态产生变化,多数细胞伸出板状伪足产生极性,细胞长度变长;相反,对照组细胞的伪足不明显,多数细胞趋于圆形,细胞直径短。划痕愈合和transwell实验显示,与对照组相比,rhBMP2诱导的MCF-7细胞的迁移和侵入能力明显加强。结论:rhBMP2能够诱导人乳腺癌细胞MCF-7获得迁移表型,并提高了MCF-7细胞的迁移和侵入能力。 AIM：To determine the effect of rhBMP2 on the migration of human breast cancer cells MCF-7. METHODS：MCF-7 was induced by rhBMP2 （30 μg/L） for 24 h. Atomic force microscopy （AFM） was used to observe the changes in cell morphology. Cell migration and invasion abilities were assayed by scratch healing and transwell experiments. RESULTS：The formation of lamellipodia and cell polarity together with increased cell length were observed in the cells treated with rhBMP2,whereas lamellipodia of cells in control group were not obvious and the majority of cells tended to be rounder with shorter cell diameter. Compared to control group,scratch healing and transwell experiments showed that the migration and invasion capacity of rhBMP2-induced MCF-7 cells was markedly enhanced. CONCLUSION：rhBMP2 induces human breast cancer cell MCF-7 to present the phenotype of migration and enhances the invasion capacity.

关 键 词： 骨形态发生蛋白质 细胞 细胞运动

领　　域： [生物学]