作　　者： ; ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《现代食品科技》 2010年第4期392-395,共4页

摘　　要： 以甲壳低聚糖(COS)平均相对分子质量为指标,研究纤维素酶以及以纤维素酶为主体与木瓜蛋白酶及果胶酶复配水解壳聚糖的最佳工艺条件。结果表明影响纤维酶水解壳聚糖因素的主次是:pH>温度>时间,最佳工艺条件为:pH=3.8,温度=60℃,时间=2.5h,可得到Mr=1467.3的甲壳低聚糖。为了适合工厂生产需要,在pH=4.4,温度=60℃,时间=2.5h条件下纤维素酶与木瓜蛋白酶及果胶酶复配,研究结果表明m(木瓜蛋白酶):m(纤维酶)=1:1时,甲壳低聚糖平均相对分子质量为1000.1,m(果胶酶):m(纤维素酶)=1/3:1时,甲壳低聚糖平均相对分子质量达1134.4。 Using th chitooligosaccharides （COS） as quality indicators, the chitosan degradation by fiber enzyme was studied. The result showed that the influence order of some key factors was： pH temperature time. And the optimum degradation conditions were as follows： temperature 60 ℃, pH 3.8, and reaction time 2.5h, under which COS was achieved with its e average molecular weight of 1467.3. For industrial production of COS, the degradation conditions were determined as pH4.4, temperature 60 ℃ and time 2.5 h. Under these conditions, the average molecular weight of COS was 1000.1 with the optimum ratio of papain to cellulose of 1：1. When the ratio of papain to cellulose was determined as 1 / 3：1, COS was achieved with its average molecular weight of 1134.4.

关 键 词： 壳聚糖 甲壳低聚糖 纤维素酶 木瓜蛋白酶 果胶酶

领　　域： [轻工技术与工程]