作　　者： ; ; ; ; ; ; ; ;

机构地区： 湖南大学环境科学与工程学院

出　　处： 《环境科学学报》 2010年第5期1002-1010,共9页

摘　　要： 采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究了农业废物堆肥过程中细菌种群随时间的变化.同时,应用Quantity One2.0和Canoco4.5软件对获得的堆肥细菌种群数据与堆肥过程因子:环境温度、堆体温度、pH、含水率、水溶性有机碳(WSC)、C/N、水溶性氨氮(NH4+-N)和硝氮(NO3--N)进行冗余分析,并做出样点、种群与堆肥过程因子的二维排序图.结果表明,细菌群落(样点)以堆肥过程因子为梯度大体可划分为升温期(1～2d)、高温期(3～11d)、降温期(12～18d)和腐熟期(19～36d)4个阶段,每一阶段均有对应种群存在.不同的堆肥过程因子对细菌种群的影响大小依次为:NO3--N>堆体温度>WSC>C/N>NH4+-N>含水率>pH>环境温度,其中,堆体温度、WSC、NO3--N、NH4+-N对细菌种群的影响极显著(p<0.01),C/N、pH对细菌种群的影响显著(p<0.05),含水率、环境温度对细菌种群的影响不显著. PCR-DGGE （Polymerase chain reaction-denaturing gradient gel electrophoresis） analysis was used to study the dynamic succession of bacterial communities during agricultural waste composting. The effects of ambient and pile temperature,pH,moisture content,water soluble organic carbon （WSC）,carbon to nitrogen ratio （C/N） and soluble NH＋4-N,NO-3-N were measured during the same time frame. Redundancy analysis （RDA） was applied to explore the relationship between bacterial species and composting parameters by using QuantityOne 2.0 and Canoco 4.5 to determine which combination of variables could explain the differences in species composition. The relationships between the ecological distribution of bacterial species and communities with the 8 parameters were clearly revealed on the RDA biplots. The composting process could be divided into four phases：mesophilic phase （days 1~2）,thermophilic phase （days 3~11）,temperature falling phase （days 12~18） and maturation phase （days 19~36）. Parameters that affect bacterial distribution and dynamic succession are in the following order：NO-3-N pile temperature WSC C/N NH＋4-N moisture content pH ambient temperature. The most important parameters significantly affecting the variation of bacterial species in agricultural waste composting were pile temperature,WSC,NO-3-N and NH＋4-N （p0.01） and C/N,pH （p0.05）. Establishing the correlation between species data and composting performance will greatly enhance the understanding and optimization of the agricultural waste composting system.

关 键 词： 堆肥 细菌群落 变性梯度凝胶电泳 冗余分析

领　　域： [环境科学与工程]