作　　者： ; ; ; ; ;

机构地区： 上海市质量监督检验技术研究院

出　　处： 《食品工业》 2010年第2期86-89,共4页

摘　　要： 采用同位素内标物定量法，建立了一种适合检测动物源性食品中三聚氰胺残留的气相色谱-质谱（GC-MS）方法。使用1％的三氯乙酸溶液提取动物源性食品中三聚氰胺残留，经阳离子交换固相萃取柱净化后，氮气吹干、硅烷化衍生，再由气相色谱-质谱联用仪检测和确证。选用同位素标记三聚氰胺（13C 15N3N3H6）内标法定量。该方法在五种具有代表性的动物食品中三聚氰胺加标回收率78．28％110．84％，相对标准偏差（RsD）不大于6．80％，在（0．02～1．00）mg／kg浓度范围内呈现良好的线性关系；灵敏度高，最低检测限达到0．023mg／kg；选择性好，能有效消除复杂基体的干扰。适合于动物源性食品中三聚氰胺的确证和定量分析。 A suitable gas chromatography-mass spectrometry （GC-MS） method was developed for the determination of melamine with isotope internal standard in animal-derived food. The melamine residue in animal- derived food was extracted by 1% solution of trichloroacetic, purified by cation exchange solid phase extraction column, blow-dried by nitrogen, derivatized by silane derivatives, and then detected and confirmed by gas chromatography-mass spectrometry, and quantified by isotope labeled melamine（13C315N3N3Hr） internal standard. With this method, the recoveries of standard melamine addition in five representative animal-derived foods ranged from 78.28% to 110.84%, each relative standard deviation（RSD） was less than 6.80%, melamine showed a good linear relationship in concentration range of （0.02-1.00） mg/kg. The detection limit was 0.023 mg/kg; and could effectively eliminate the interference of the complex ground substance. The method is suitable for conformation and quantitative determination of melamine in animal-derived food.

关 键 词： 同位素内标 气相色谱 质谱 三聚氰胺 同位素标记三聚氰胺 动物源性食品

领　　域： [理学] [理学]