作　　者： ; ; ; (高洪贵）;

机构地区： 山东轻工业学院轻化与环境工程学院制浆造纸科学与技术教育部重点实验室

出　　处： 《中华纸业》 2010年第8期30-34,共5页

摘　　要： 具有5-5′结构的木素结构非常稳定,难以在制浆过程和废水处理中降解,但酚型5-5’结构能在漆酶的作用下发生聚合。为了了解在漆酶的催化作用下5-5’结构型木素结构与对-羟基肉桂酸之间的共聚性能,进而为制浆废水中木素的酶催化絮凝技术的开发提供理论基础,本文研究了在漆酶催化作用下,对-羟基肉桂酸与5-5’结构木素模型物(脱氢二香草醇)的聚合反应。应用凝胶渗透色谱(GPC)测定了聚合产物的相对分子量,采用红外光谱、核磁共振技术探讨了对-羟基肉桂酸与5-5′结构木素模型物的脱氢聚合产物的化学结构。研究结果表明:在漆酶催化作用下,对-羟基肉桂酸与5-5′结构木素模型物发生脱氢聚合反应,形成典型的DHP。聚合后相对分子质量明显增大,相对分子量Mn由306提高到2106。结构单元之间的连接方式主要是通过β-O-4、α-O-4、β-β等。 The 5-5′ type lignin substructure is very stable during kraft pulping and it is difficult to degrade in the treatment of wastewater from pulping. However, phenolic 5-5′ can polymerize by laccase. In order to understand the co-polymerization between 5-5′ type lignin model and p-hydroxycinnamic acid in existence of laccase to provide the theoretic basis for the development of precipitation and flocculation of the lignin in effluent from pulping process, the polymerization reaction between p-hydroxycinnamic acid and a lignin model compound of phenolic 5-5′ type lignin model compound, i.e. dehydrodiacetovanillione, catalyzed by laccase was studied .Gel permeation chromatography （GPC） was used to analyze the molecular weights of the polymerization products. FT-IR and NMR spec-troscopy were employed to determine the chemical structure of the polymer obtained. The result indicates that dehydrodivanillyl alcohol can polymerize with p-hydroxycinnamic acid catalyzed through dehydrogenation. While GPC determination indicated the molecular weight Mn increased from 306 to 2106. The linking ways of structure units were mainly though β-O-4、α-O-4、β-β structures.

关 键 词： 漆酶 对 羟基肉桂酸 结构 脱氢聚合

领　　域： [轻工技术与工程] [轻工技术与工程]