作　　者： ; ; ; ; ;

机构地区： 西北农林科技大学动物科技学院

出　　处： 《中国兽医学报》 2010年第3期402-405,共4页

摘　　要： 建立家兔血清中苦马豆素(SW)浓度的气相色谱测定方法,为SW的毒理学和免疫学研究提供条件。饲喂家兔甘肃棘豆草粉后,分别于5、10、20d采集血液并分离血清;血清经过丙酮沉淀、超声和离心净化处理后,冷冻干燥;冻干物经硅烷化试剂衍生化反应,用气相色谱内标法测定血清中SW的含量。结果显示,测定SW的线性范围为0.00008～2.5g/L,相关系数γ=0.9996,检出限为0.00008g/L。在0.001、0.01、0.1、1.0g/L分别添加SW的回收率为89.81%～96.23%,相对标准偏差为2.46%～4.35%。结果表明,该方法准确、灵敏、快速、简便,适用于家兔血清中SW含量的测定。 A gas chromatographic（GC） method was established to determinate the concentration of swainsonine in the rabbits serum,which created the conditions for the next immunological study.The rabbit serum was collected in the 5 th,10 th and 20 th day afer feeded Oxytropis kansuensis,respectively,acetone was added in the serum samples,then the Vacuum Freezy-desiccation were used after purified by ultrasonic wave and centrifuging.Then the concentration of swainsonine in the serum samples were analyzed by gas chromatographic internal standard method after derivatization with slilylreagents.The standard curve was linear within the investigated range of 0.000 08-2.5 g /L.The correlation coefficient was 0.999 6.The detection limit was 0.000 08 g /L.The recoveries were 89.81%-96.23% with the relative standard deviations of 2.46%-4.35% at the spiking levels of 0.001-1.0 g /L.The method is accurate,sensitive,rapid and simple,it could be used to determinate the serum concentration of swainsonine in the rabbit.

关 键 词： 苦马豆素 血清 气相色谱 衍生化

领　　域： [农业科学] [农业科学] [农业科学]