作　　者： ; ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《海洋湖沼通报》 2010年第1期52-58,共7页

摘　　要： 报道了卵形鲳鲹(Trachinotus ovatus)诺卡氏菌病的发生情况。病鱼以体表和心、脾、肾等内脏出现白色结节为主要症状。对病鱼进行细菌分离培养和光镜、电镜检查,均发现长或短的丝状分枝状杆菌,用分离得到的菌株进行回归感染,证实为此次卵形鲳鲹结节病的病原菌。对病原菌的基本生物学特性进行了研究,并结合分子生物学方法进行鉴定,确定病原为鰤鱼诺卡氏菌(No-cardia seriolae),同时对病原菌进行药物敏感检测,发现该病原菌对多种常用水产药物均产生了抗药性,纸片扩散法结果显示仅20种受试药物中仅对环丙沙星、氯霉素、链霉素等中度敏感,试管稀释法结果显示8种药物中仅对1×10-6的盐酸恩诺沙星和盐酸环丙沙星敏感。 An epizootic in seawater cage-reared Trachinotus ovatus in China was reported in this paper.White nodules were scattered on the heart,spleen and kidne of diseased fish.The morphology of isolated bacteria from Lowenstein-Jensen medium（LJM） was Gram-positive branching,bead-like or long,slender,filamentous rods.Experimental infection indicated that the isolated bacterium was pathogen responsible for mortalities.Based on the symptom of diseased fish and the growth characteristics,morphological,physiological,and molecular biological properties of the bacterium,the isolated were identified as Nocardia seriolae.Antibiotic testes for Nocardia seriolae was also carried out.The results showed that it was resisted to many actibiotics,and was just sensitive to profloxacin,chloramphenicol,streptomycin,enrofloxacin hydrochloride,and profloxacin hydrochloride.

关 键 词： 卵形鲳鲹 结节病 鰤鱼诺卡氏菌

领　　域： [农业科学] [农业科学]