作　　者： ; ; ; ; ; ;

机构地区： 中国农业科学院蜜蜂研究所

出　　处： 《中国农业科学》 2010年第8期1703-1715,共13页

摘　　要： 【目的】对原种意大利蜜蜂(意蜂)和原种意大利蜜蜂的王浆高产品系(浆蜂)工蜂蛹期头部的蛋白质组进行比较,以探明浆蜂和意蜂3个日龄(13、15、17日龄)蛋白质表达调控方面的差异,为阐明蜜蜂发育生物学提供一定理论基础。【方法】采用双向电泳建立浆蜂和意蜂3个日龄蛹期头部蛋白质组表达谱,获得图谱中蛋白质的种类、表达量、等电点和分子量等信息,并通过质谱分析、数据库检索,鉴定部分差异蛋白。【结果】在13日龄蛹头部,浆蜂表达279个蛋白,意蜂表达268个蛋白,浆蜂和意蜂共有蛋白为212个,而浆蜂和意蜂的特异蛋白分别为67个和56个;到15日龄时,浆蜂表达296个蛋白,意蜂为277个,浆蜂和意蜂共有蛋白点为203个,浆蜂特异蛋白点为93个,意蜂特异蛋白点为74个;到17日龄蛹时,浆蜂蛋白质组有340个蛋白点,而意蜂则有279个蛋白点,浆蜂和意蜂共有蛋白点为246个,浆蜂的特异蛋白点为94个,意蜂特异蛋白点为33个。对部分差异蛋白质进行质谱分析后,鉴定出11个蛋白质。对已鉴定蛋白质表达量聚类分析,结果聚为2类:表达量上调的分别是微管蛋白、肌动蛋白88F、ATP合成酶、谷胱甘肽S-转移酶S1、精氨酸激酶、热激蛋白、蛋白酶体2亚基、泡液ATP合成酶催化亚基和转录起始因子5A;表达量下调的蛋白质为过氧化物氧化还原酶2540。【结论】浆蜂经过蜂王浆高产的选育,表达的蛋白质种类比意蜂增加。随着日龄的增长,大多数与细胞骨架、抗氧化相关和代谢相关的蛋白质显著上调表达,表明工蜂蛹头部的王浆腺和唾液腺等组织在生长发育,新陈代谢加强以及神经系统逐渐重塑。这将对全面理解浆蜂和意蜂蛹期发育规律奠定基础。 [Objective] To investigation into the differentially expressed proteins between the higher royal jelly producing （HRJB） and the native Italian honeybee （NIB） （Apis mellifera L.）, the proteome profilings of the worker pupal head of the two honeybee strains were compared on 3 time-points （day 13, 15, 17） for providing a theoretical principle of clarifing honeybee developmental biology. [Method] Two-dimensional gel electrophoresis （2-DE） was employed to establish the proteomic map of the worker pupal head of the two honeybee strains on 13, 15 and 17 days, then the protein expression abundance, molecular weight and pI was obtained. Part of differentially expressed proteins was identified by analysis of matrix-assisted laser desorption/ionization-time of flight mass spectrometry （MALDI-TOF/MS） and database research. [Result] A total of 279, 296 and 340 proteins were detected on 3 time-points in HRJB, and 268, 277 and 279 were observed in NIB, respectively. A significantly higher protein spot numbers were observed in HRJB than those in NIB. Two hundred and twelve proteins were presented in the worker pupal head of these two bees on day 13, 67 proteins were specific in the worker pupal head of HRJB and 56 proteins were exclusive to NIB. While on day 15, 203 shared proteins and 93, 74 unique proteins were detected in HRJB, NIB, respectively. Until dayl7, 246 proteins were resolved in the head of both bees strains in the pupal period, 94 and 33 proteins were found unique to the worker pupal head of the HRJB and NIB, respectively. During the determination of PMF, 11 proteins were unambiguously identified. Hierarchical cluster analysis divided the 11 proteins into two categories, i. g. tubulin, actin 88F, ATP synthase, glutathione S transferase S 1, arginine kinase, heat shock protein, proteasome 2 subunit, vacuolar ATP synthase catalytic subunit A and eukaryotic translation initiation factor 5A were up-regulated their expression, while only peroxiredoxin 2 540 was down-regulated in the head during t

关 键 词： 原种意大利蜜蜂 王浆高产蜜蜂 双向电泳 蛋白质组 蛹期

领　　域： [农业科学]