作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院农药与化学生物学教育部重点实验室

出　　处： 《中国农业科学》 2010年第8期1601-1607,共7页

摘　　要： 【目的】研究α-三联噻吩（α-T）对斜纹夜蛾（Spodoptera litura）SL细胞线粒体膜电位及细胞周期的影响。【方法】通过Rhodamine123染色和流式细胞术（flow cytometry,FCM）研究α-T处理SL细胞24 h和48 h后细胞线粒体膜电位的变化,并通过PI染色和FCM,测定α-T处理SL细胞24 h和48 h后细胞周期各时相百分率的变化。【结果】光活化后的α-T处理SL细胞24 h后,0.0625 μg·mL^-1浓度和0.1250 μg·mL^-1浓度处理组细胞线粒体膜电位的变化幅度不大,而0.5000 μg·mL-1浓度处理组细胞线粒体膜电位明显升高,48 h后高浓度处理组（1.0000 μg·mL^-1）导致线粒体膜电位明显去极化;处理24 h后,低浓度（0.0625 μg·mL^-1）处理组细胞被阻滞于S期,而高于此浓度的光照处理组细胞被阻滞于G2/M期,48 h后,浓度≤0.1250 μg·mL^-1的光照处理细胞被阻滞于G2/M期,而浓度＞0.1250 μg·mL^-1的光照处理组细胞被阻滞于S期。【结论】SL细胞经α-T处理后,细胞处于一系列复杂的动态变化中。当细胞本身不能扭转ROS造成的氧化损伤时,细胞线粒体膜电位和细胞周期时相即出现较大幅度的变化,这种变化决定着细胞的受损程度和细胞的死亡途径。 [ Objective ] To obtain the influence of α-T on the mitochondrial membrane potential and cell cycle of insect cell is essential. [Method] Rhodamine123 and flow cytometer were employed to acquire the influence of α-T on the mitochondrial membrane potential of Spodoptera litura cell after 24 h and 48 h treatments. The cell cycle phases were determined with propidium iodide （PI） through FCM. [Result] After 24 h treatment, the mitochondrial membrane potential of cells treated with 0.0625 μg·mL^-1 and 0.1250 μg·mL^-1 changed little. While the mitochondrial membrane potential of cells treated with 0.5000 μg·mL^-1 increased obviously. After 48 h, the mitochondrial membrane potential of the treatment with high dose （1.0000 μg·mL^-1） depolarized. Moreover, the cell cycle was arrested in S phase when SL cell was treated with low dose （0.0625 μg·mL^-1） after 24 h treatment. However, it was arrested in G2/M phase when treated with higher dose. After 48 h, the cell cycle was arrested in G2/M phase with lower dose （≤0.1250 μg·mL^-1） and in S phase with higher dose （≥0.1250 μg·mL^-1）. [Conclusion] After treatment, the cells were in a serial complicated status. When the ceils couldn＇t resist the oxidation damage caused by ROS, the mitochondrial membrane potential and cell cycle phase changed violently. These changes decided the path of cells damage and death.

关 键 词： 三联噻吩 斜纹夜蛾 细胞 线粒体膜电位 细胞周期 流式细胞术 碘化丙啶

分 类 号： [S433.4]

领　　域： [农业科学] [农业科学]