机构地区: 广西大学化学化工学院
出 处: 《广西科学》 2010年第1期68-71,共4页
摘 要: 结合均匀设计试验方法及数据挖掘技术,采用紫外-可见吸收光谱及共振散射光谱研究铬天青Cu(Ⅱ)配合物与牛血清白蛋白的相互作用。确定试验的最佳条件为pH值=6,0.1mol/L离子强度剂(NaCl)用量0.5ml,0.1%铬天青用量0.6ml。得到紫外-可见吸收光谱的吸光度随着铜离子浓度的增加而线性下降,其对应的方程及参数为:ΔA=0.08302-0.06207C,R=-0.9987,SD=0.001830。共振散射光谱的强度则随着铜离子浓度的增加而线性的增强,其对应的方程及参数为:ΔI=-226.15+287.9C,R=0.9866,SD=17.38。 The interaction between CAS-Cu(Ⅱ) complex and bovine serum albumin(BSA) was studied with UV absorption spectroscopy and resonance scattering spectroscopy.Uniform experimental design as well as data mining technology were applied in the experiments,in which the optimal technical conditions were obtained.The experimental results of the optimal technical conditions showed that the absorbency of UV absorption spectra linearly decreased with increase of Cu(Ⅱ) concentration.The linear equation and parameters were:ΔA=0.08302-0.06207C,R=-0.9987,SD=0.001830,and the intensity of resonance scattering spectra linearly increased with increase of Cu(Ⅱ) concentration.The linear equation and parameters were:ΔI=-226.148+287.9015C,R=0.9866,SD=17.38261.