作　　者： ; ; ; ; ;

机构地区： 中国科学院海洋研究所海洋地质与环境重点实验室

出　　处： 《地球科学（中国地质大学学报）》 2010年第2期254-260,共7页

摘　　要： 为研究东海内陆架闽浙沿岸泥质区不同深度沉积物中古菌群落垂向分布特征,利用古菌16SrDNA基因文库得到473个有效克隆、50个OTUs(Operational Taxonomic Units).16SrDNA序列系统进化和统计分析发现古菌分别归属于泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota),其中以Miscellaneous Crenarchaeotic Group(MCG)为主,仅含少量的Marine Benthic GroupB(MBG-B)、South African Gold Mine Euryarchaeotic Group(SAGMEG)、Anaerobic Methanotrophs3(ANME-3)、Marine Crenarchaeotic GroupI(MG-I)和Marine Benthic GroupD(MBG-D).该泥质区沉积物可能存在由ANME-3催化的甲烷厌氧氧化作用,同源序列分析表明其古菌群落分布与周边环境有较大联系.UniFrac与沉积物环境因子分析表明该泥质区古菌群落垂向分布与沉积物有机质含量和粒度变化密切相关. Archaeal 16S rDNA clone libraries were constructed from sediments of different depths to elucidate the distribution and diversity of archaea in the Fujian and Zhejiang coastal mud wedge of the inner continental shelf of the East China Sea.We obtained 473 useable clones and 50 different OTUs （Operational Taxonomic Units）.Phylogenetic and statistic analysis of clone libraries show that：archaeal 16S rDNA sequences are within phylums of Crenarchaeota and Euryarchaeota,respectively.The majority of archaeal phylotypes are Miscellaneous Crenarchaeotic Group （MCG）,whereas few sequences fall into Marine Benthic Group B （MBG-B）,South African Gold Mine Euryarchaeotic Group （SAGMEG）,Anaerobic Methanotrophs 3 （ANME-3）,Marine Crenarchaeotic Group I （MG-I） and Marine Benthic Group D （MBG-D）.There might be anaerobic oxidation of methane carried out by group ANME-3 in the mud wedge.Homologous sequence analyses show that the distribution of archaea in the mud wedge is related to the surrounding environment.UniFrac analysis and sediment environmental parameters indicate that the vertical distribution of archaea closely coincides with the variation of sediment total organic carbon content and grain size.

关 键 词： 内陆架 东海 泥质区 古菌 垂向分布 生物多样性

领　　域： [天文地球] [天文地球]