作　　者： ; ; ; ; ; ; ;

机构地区： 中国海洋大学水产学院海水养殖教育部重点实验室

出　　处： 《中国海洋大学学报（自然科学版）》 2010年第3期71-77,共7页

摘　　要： 采用巢式RT-PCR法克隆了半滑舌鳎Cynoglossus semilaevis促黄体激素受体(Luteinizing Hormonene Recep-tor,LHR)基因部分序列,经过BLAST比对,认为该序列与庸鲽、牙鲆、黑鲷、尼罗罗非鱼、青鳉和红鳍东方鲀的LHR基因同源性分别为97%,94%,85%,83%,82%和80%。该基因片段长度为614 bp,编码204个氨基酸,含有典型的跨膜螺旋结构区域(TM helix),属于糖蛋白激素受体(GHR)家族。组织表达分析表明,LHR基因在半滑舌鳎的卵巢、精巢、肝脏、胃、肠、鳃、心、脾、肾、头肾和脑中均有表达,表达量有所不同,以脾和肾中表达量最丰富。 Partial cDNAs encoding LHR（luteinizing hormonene receptor） gene were cloned from Cynoglossus semilaevis by nested reverse transcrip-polymerase chain reaction（nested RT-PCR）.Alignment analysis showed that the sequence was 97%,94%,85%,83%,82% and 80% similar to those of Hippoglossus hippoglossus,Paralichthys olivaceus,Acanthopagrus schlegelii,Oreochromis niloticus,Oryzias latipes and Takifugu rubripes,respectively.The length of the fragment is 614 bp,and encodes a protein of about 204 amino acids,which contains the conserved seven transmembrane helix domains（TM helix）,and it belongs to the glycoprotein hormone receptor（GHR）family.Tissue expression analysis showed that LHR mRNA expressed in all eleven tissues including ovary,spermary,liver,stomach,intestines,gill,heart,spleen,kidney,head kidney,and brain,from which the spleen and kidney presented the strongest amplification signals.

关 键 词： 半滑舌鳎 促黄体激素受体 巢式 组织表达

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]