作　　者： ; ; ; ;

机构地区： 华南师范大学生命科学学院

出　　处： 《华南师范大学学报（自然科学版）》 2010年第1期98-104,共7页

摘　　要： 通过比较几种常用的化学激活方法对小鼠卵母细胞的孤雌激活和孤雌生殖胚胎体外发育的影响,从而筛选出小鼠卵母细胞孤雌激活的适宜方法.选取成熟小鼠卵母细胞,随机分为以下9组进行激活处理:①离子霉素(I)+6-二甲氨基嘌呤(D);②I+D+细胞松弛素B(CB);③I+放线菌酮(C);④I+C+CB;⑤φ(乙醇)=7%的乙醇(E)+D;⑥E+D+CB;⑦E+C;⑧E+C+CB;⑨Sr2++CB.比较了这9种化学激活方法以及SrCl2的作用时间和卵龄对小鼠卵母细胞激活效果的影响.实验结果表明:小鼠卵母细胞孤雌激活率与激活措施和卵龄有关;小鼠卵母细胞可以被I+D+CB或SrCl2+CB有效激活,激活率及孵化囊胚发育率均高于其他激活方法(P<0.05),且以SrCl2+CB最为有效.hCG注射后20h为小鼠卵母细胞进行SrCl2激活的最适卵龄,其最佳处理时间为4h.对小鼠卵母细胞而言,6-DMAP比放线菌酮的激活效果好. In order to find out the suitable activation protocol for mouse oocytes, the cleavage and development of mouse oocytes activated by several different chemical activation protocols were investigated. Matured oocytes were grouped into 9 treatments randomly： （1） inomycin ＋ 6-dimethylaminopurine; （2） ionomycin ＋ 6-DMAP＋ cytochalasin B （CCB）; （3） ionomycin＋ cycloheximide （CHX）; （4） ionomycin ＋ CHX ＋ CCB; （5） 7% ethanol ＋ 6-DMAP; （6） 7% ethanol ＋ 6-DMAP ＋ CCB; （7） 7% ethanol ＋ CHX; （8） 7% ethanol ＋ CHX ＋ CCB; （9） Sr2＋ ＋ CCB. The parthenogenetic efficiencies of these activation protocols on mouse oocytes, the optimum treatment duration and the age of oocytes activated by Sr2＋ ＋ CCB were analysed. The results indicated that the parthenogenetic efficiencies of mouse oocytes are related with activation protocols and the age of oocytes. Mouse oocytes can be activated effectively by ionomycin ＋ 6-DMAP ＋ cytochalasin B or Sr2＋ ＋ CCB, and the rate of activated oocytes and the development rate of hatched blastocysts in these groups are higher than that in other treatment groups （P0.05）. Sr2＋ ＋ CCB protocol is the suitable method for mouse oocyte activation. Mouse oocytes at 20h post hCG injection are the optimum stage for parthenogenetic activation by Sr2＋ ＋ CCB and 4h is the best treatment duration for Sr2＋ ＋ CCB activation. 6-DMAP is more effective than CHX in activating mouse oocytes.

关 键 词： 卵母细胞 化学激活 激活方法 小鼠

领　　域： [生物学]