机构地区: 河北农业大学食品科技学院
出 处: 《中国酿造》 2010年第3期132-135,共4页
摘 要: 通过对于透明质酸产生菌发酵培养基,利用Plackett-Burman设计及响应面试验分析了摇瓶培养基成分,确定了最适合的摇瓶发酵培养基。通过小型发酵罐的研究确定了透明质酸的发酵工艺。实验结果表明,利用响应面实验优化的培养基组分简单。透明质酸的产量达到5.23g/L,分子量为2.0×106u。 The optimal medium of hyaluronic acid (HA) fermentation produced by Streptococcus zooepidemicus was investigated by Plackett-Burman design and Response surface design in shaking flask. The optimal fermentation conditions were obtained in fermentator. The results showed that the medium composition in shaken-flask fermentation was simple. The yield of HA and molecular weight were 5.23 g/L and 2.0×10^6 Da, respectively.
领 域: [生物学]