作　　者： ; ; ; ;

机构地区： 郑州大学化工与能源学院

出　　处： 《郑州大学学报（工学版）》 2010年第1期74-77,共4页

摘　　要： 秸秆直接还田会给作物生长带来不利影响,在还田过程中补加纤维素降解菌剂是解决其不利影响的有效方法.通过平板初筛及酶活综合测定及秸秆降解率测定复筛,从腐烂秸秆、牛粪堆肥、森林土等样品中筛选到5株高效纤维素降解菌,其中X1菌的酶活最佳,酶系组成最合理,其Cx,Cb,C1和FPA分别达到53.6 U/mL、69.7 U/mL、9.5 U/mL和11.6 U/mL,对秸秆的降解率达40.2%.进一步进行菌种组合,得到了一组秸秆降解率有明显增加的复合菌X1+X2,其降解率达到45.5%.根据X1和X2的菌落及个体形态特征,初步判定其均为青霉菌. Straw returning to farmland directly has negative effects on seed germination and crop growth.Adding high-efficient cellulose-decomposing bacteria is an effective method to resolve this problem.Five strains with different cellulase composition were isolated from rotten straw,cow dung compost,and paddy soil.One of the five strains which named X1 had the maximum cellulase activity and the optimal cellulase composition.In this study the Cx,Cb,C1and FPA of X1 were 53.6 U/mL、 69.7 U/mL、 9.5 U/mL and 11.6 U/mL,and the straw degradation efficiency was up to 40.20%.The degradation efficiency of the combination of strains X1 and X2 was up to 45.50%,which higher than the others combination strains.According to the morphological characteristics of X1 and X2,it was deduced that they were Penicillium.

关 键 词： 秸秆还田 纤维素降解 筛选 菌种组合

领　　域： [农业科学] [农业科学] [化学工程]