作　　者： ; ; ; ; ; ; ;

机构地区： 华南农业大学兽医学院农业部动物疫病防控重点开放实验室

出　　处： 《畜牧兽医学报》 2010年第2期193-199,共7页

摘　　要： 本研究从临床表现为典型血管瘤型禽白血病病例的广东某肉种鸡场的病鸡中,分离到1株J亚群禽白血病病毒(ALV-J),命名为ZH-08。利用ELISA抗原检测、PCR和间接免疫荧光试验对分离株进行鉴定,结果都呈阳性。依据ALV-J原型株HPRS-103前病毒全基因组序列设计并合成3对引物,采用分段扩增的方法完成了分离株的全基因组序列测定。结果显示该分离株基因组序列全长7 597 bp,与已公开的全基因组序列大小比较略有差异,但符合典型的复制完全型反转录病毒的基因组结构,基因序列中不含已知致癌基因。将该分离株的亚群特异性gp85基因序列与国内外各参考株相应序列进行相似性比较,发现ZH-08与YZ9901株相似性最高(93.7%)。基于gp85核苷酸序列的系统进化分析表明:ZH-08株与SD07LK1株的亲缘关系最近。本研究为该毒株的生物学特性以及致病机制研究奠定了基础。 A subgroup J avian leukosis virus（ALV-J）,designated as ZH-08,was isolated from broiler breeder flock with a novel hemangioma case in Guangdong Province.The identified results of ELISA test,PCR and immunofluorescence assay（IFA） specific for ALV-J,were all positive.Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103,three pairs of primers were synthesized,and the full-length proviral genome of ZH-08 isolate was sequenced by the method of fragment amplification.The full-length proviral genome sequence of ZH-08 isolate is 7 597 bp,its size has a little difference from that of published full-length genome sequences,but its structure corresponds with typical retroviral genome structure for completely copy ability,the known oncogenes were not included in its genome.According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains at home and abroad,the similarity with YZ9901 isolate was the highest（93.7%）.Phylogenetic analysis based on gp85 gene showed that ZH-08 isolate had the closest linkage to SD07LK1 isolate.This study provide the basis of biological characteristics and pathogenesis research of ZH-08 isolate.

关 键 词： 血管瘤 亚群禽白血病病毒 全基因组序列

领　　域： [农业科学] [农业科学] [农业科学]