作　　者： ; ; ; ; ;

机构地区： 四川理工学院生物工程学院

出　　处： 《生物技术》 2010年第1期81-84,共4页

摘　　要： 目的:提高透明质酸的纯度。方法:研究了絮凝预处理发酵液对醇沉法提取透明质酸的影响,并通过正交试验对预处理条件进行了优化。结果:明矾可作为最佳絮凝剂,正交实验最佳絮凝条件为:絮凝剂添加量1 000mg/L、絮凝温度30℃、絮凝转速60r/min、絮凝时间20min。在该条件下处理的HA发酵液相对于未经过处理的对照组,菌体去除率可提高42.6%,蛋白去除率可提高5.9%。 Objective： Taking the improvement of the purity. Method： The effect of purification of Hyaluronic acid from broth by ethanol precipitation method was investigated by pretreating the broth using flocculants,and the factors were optimized by the orthogonal method. Result： Alum is the best flocculants. The best flocculating condition is got by orthogonal experiment. A conclusion could be got that tbe removal rate of cell could be improved by 42.6% and The removal rate of protein could be improved by 5.9% under the condition of addition 1000 mg/L, temperature 30℃, flocculating speed 60r/min and flocculating time 20min.

关 键 词： 透明质酸 发酵液 分离提纯 絮凝

领　　域： [生物学]