作　　者： ; ; ; ; ; ; ;

机构地区： 广东省实验动物监测所

出　　处： 《中国比较医学杂志》 2010年第2期78-82,共5页

摘　　要： 目的了解广东省实验小鼠自然感染小鼠诺如病毒(murine norovirus,MNV)的情况。方法随机抽取广东省7个繁育设施的小鼠206只,应用逆转录-聚合酶链反应(RT-PCR)方法检测其感染MNV的情况。结果共检测小鼠盲肠内容物206份,阳性样本为77份,阳性率为37.38%。3个设施的小鼠感染MNV,各品系小鼠易感性差异无显著。结论证实广东省小鼠存在MNV感染,部分设施小鼠MNV感染率很高,需加强动物的饲养管理。RT-PCR方法可以应用于MNV感染检测。 Objective To investigate the natural infection rate of murine norovirus（MNV）in laboratory mice in Guangdong province.Methods／206 Mice were selected randomly from seven mice breeding facilities in this province for a survey of MNV infection rate.Reverse transcription-polymerase chain reaction（RT-PCR） was used to detect MNV in the mouse cecal contents.Results 206 samples were tested and 77 samples were positive for MNV,the infection rate was 37.38%.The mice of three facilities were infected with MNV.There were no significant differences in susceptibility between all tested mice strains.Conclusions／It is confirmed that MNV infection exists in laboratory mice in Guangdong,and the infection rate is very high in some animal facilities.It is necessary to enhance the animal breeding management.RT-PCR can be used to detect MNV infection in mice.

关 键 词： 鼠诺如病毒 逆转录 聚合酶链反应 自然感染

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]