作　　者： ; ; ; ;

机构地区： 仲恺农业工程学院农业与园林学院

出　　处： 《核农学报》 2010年第1期20-24,共5页

摘　　要： 采用AFLP技术对辣椒胞质雄性不育系‘北A’、相应保持系‘北B’及其杂种F1的m tDNA进行了比较分析,获得了9条差异谱带,对全部差异谱带进行了克隆测序和同源性比对分析。结果表明,部分序列同烟草NADH脱氢酶亚基1和核糖体蛋白L2基因、矮牵牛线粒体嵌合基因nad4L-orf25等核苷酸序列存在90%以上的相似性。推断这些差异片段可能与辣椒细胞质雄性不育相关。 Mitochondrial DNA（mtDNA） from pepper CMS line ＇North A＇,maintainer line ＇North B＇ and their hybrid F_1 were analyzed and compared by AFLP technique.9 specific fragments were obtained,and were cloned and sequenced.BLAST analysis in GenBank revealed that partial sequences shared above 90% identity with NADH dehydrogenase subunit 1,ribosomal protein L2 of Nicotiana tabacum,nad4L-orf25 intergenic sequence of Petunia×hybrida mitochondrial DNA at nucleotide level.It was speculated that the specific fragments might be related to CMS in pepper.

关 键 词： 辣椒 细胞质雄性不育 线粒体

领　　域： [农业科学] [农业科学] [生物学]