作　　者： ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品研究与开发》 2010年第2期155-159,共5页

摘　　要： 建立一种快速、准确检测变形杆菌属的PCR方法。选择tuf基因作为靶基因设计引物,分别对3株变形杆菌属标准菌株、66株变形杆菌属样品分离株及12株非变形杆菌属菌株进行扩增试验,结果3株标准菌株和66株样品分离株均扩增出541bp的特异性片断,12株非变形杆菌属菌株均未有特异性条带产生,呈阴性。灵敏度试验结果表明:该法检测限可低至1.08×103cfu/mL。该方法可用于食品中变形杆菌属的快速检测。 A quick and accurate method of Polymerase Chain Reaction （PCR） for detection of Proteus was established. A pair of primers were designed by taking the tuf gene as objective sequences to amplify the genes of 3 references Proteus, 66 isolated Proteus and 12 non-proteus strains. Expected 541 bp fragments were obtained for 3 references Proteus and 66 isolated Proteus. No specific fragments were detected in the 12 strains of the non-Proteus bacteria. The result of specificity tests showed that the detection limit could reach 1.08×10^3 cfu/mL. It＇s applicable of detecting the pathogenic Proteus rapidly in food.

关 键 词： 食源性致病菌 变形杆菌属 聚合酶链反应 检测

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]