作　　者： ; ;

机构地区： 华南农业大学园艺系

出　　处： 《华南农业大学学报》 1998年第4期61-66,共6页

摘　　要： 比较了青花菜不同外植体对分化不定芽的影响，结果表明，在ＭＳ添加吲哚乙酸（ＩＡＡ）１．０ｍｇ／Ｌ和６－苄基腺嘌呤（ＢＡ）２．０ｍｇ／Ｌ培养基上，子叶、下胚轴、花序柄及花蕾的不定芽分化率分别为５０％、１００％、１４％和７３％；在各种外植体所分化形成的不定芽中，除花蕾所分化的不定芽有７４％表现正常外，其余的不定芽几乎玻璃化与此同时，以下胚轴作为外植体，探讨玻璃苗抑制途径的初步结果显示，提高培养基琼脂质量浓度至１１．０～１４．０ｇ／Ｌ以及三角瓶用棉塞和牛皮纸作为封口材料时，有利于正常不定芽的分化形成；而提高培养基中蔗糖浓度或培养基的渗透压。 The effect of different explants of broccoli on shoot regeneration was compared.The results showed, on the medium of MS containing indol 3 acetic acid 1.0 mg/L and 6 benzyladenine 2.0 mg/L, the frequency of shoot regeneration from cotyledon,hypocotyl, inflorescence stem and flower bud was 50%, 100%, 14% and 73% respectively. All of the shoots regenerated from various explants, except that 74% of the shoots from flower bud were normal, were nearly vitrified. Meanwhile,the ways for overcoming vitrification using hypocotyl as explant was studied.The preliminary results showed that the normal shoot regeneration was facilitated by increasing mass concentration of agar to 11.0～14.0 g/L in medium, or covering the flask with cotton plug and kraft paper. But there were no evident effect for inhibiting the occurrence of vitreous shoot by increasing sucrose concentration or osmotic pressure, changing the pH and supplementing chlorochloline chloride (CCC) in medium.[KH+2mmD]

关 键 词： 青花菜 离体培养 玻璃苗 试管苗 玻璃化

领　　域： [农业科学] [农业科学]