作　　者： ; ;

机构地区： 广东省农业科学院

出　　处： 《中国兽医杂志》 1998年第12期5-7,共3页

摘　　要： 利用超速离心纯化的鸡传染性支气管炎病毒（ＩＢＶ）广东地方分离株Ｄ４１免疫ＢＡＬＢ／ｃ小鼠。取脾细胞与ＳＰ２／０骨髓瘤细胞进行融合，经筛选及克隆，成功地建立了６株可分泌抗ＩＢＶＤ４１株的单克隆抗体（ＭｃＡｂ）的杂交瘤细胞：Ｂ５、Ｂ８、Ｃ７、Ｄ８、Ｄ９、Ｇ１１。其中Ｂ８、Ｃ７、Ｇ１１的稳定性最好，腹水ＭｃＡｂ效价高达１０１０，无血清培养上清ＭｃＡｂ（浓缩３０倍）效价达４×１０４以上。６株杂交瘤细胞的特异性均很好。 s In this study,avian infectious bronchitis virus(IBV)strain D 41 isolated from Guangdong province was used for antigen and purified by sucrose density gradient centrifugation. The monoclonal antibodies(McAbs)were generated by fusing Sp2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with the antigen. After screening and cloning,six murine hybridoma cell strains (B 5、B 8、C 7、D 8、D 9、G 11 )which secrete specific McAbs to IBV strain D 41 were successfully established. Three of the six hybridomas(B 8、C 7、G 11 )are more stable.Their ascitic fluids titers and serum-free culture fluids(condensed 30 times titers were more than 10 10 ×10 4 respectively.

关 键 词： 鸡 传染性支气管炎 病毒 单克隆抗体 杂交瘤细胞

领　　域： [农业科学] [农业科学] [农业科学]