作　　者： ; ; ;

机构地区： 南昌大学生命科学与食品工程学院食品科学与技术国家重点实验室

出　　处： 《生物加工过程》 2010年第1期66-71,共6页

摘　　要： 利用响应面法优化超临界CO2萃取苦瓜总黄酮的工艺参数,在单因素实验基础上,以萃取时间、萃取温度及萃取压力为自变量,总黄酮提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对总黄酮提取率的影响。结果表明,3个因素对总黄酮提取率的影响大小依次为萃取压力、萃取温度、萃取时间。利用SAS软件和响应面分析相结合的方法模拟得到二次多项式回归方程的预测模型,确定最佳工艺条件:采用无水乙醇为夹带剂(4.0 mL/g),萃取压力33.4 MPa、萃取温度46℃、萃取时间53.2 m in。此条件下,苦瓜总黄酮提取率达到84.3%。抗氧化实验表明:超临界CO2萃取能较好保留苦瓜总黄酮的抗氧化活性,采用超临界CO2萃取法提取的苦瓜总黄酮具有较强的抗氧化活性,当质量浓度为1 mg/mL时,对DPPH自由基的清除能力与Vc相当,清除率达到93.1%。 Response surface methodology was used to determine the optimum processing conditions for su- percritical carbon dioxide extraction of flavonoids from Momordica charantia L. Based on single factor ex- periments, the effects of pressure, temperature, and extraction time were studied with the central com- posite design and their interactions on the yield of flavonoids were also investigated. The influence of the order was pressure, temperature, and extraction time. The polynomial quadratic equation was solved by SAS software combined with the response surface methodology. Optimum conditions were pressure 33.4 MPa, temperature 46 ℃, extraction time 53.2 min, and absolute ethanol as entrainer on as follows：the ratio of 4. 0 mL/g. The extraction rate was up to 84. 3 %. The scavenging ability on 1,1-diphenyl-2 - picrylhydrazyl reached 93.1%, as referenced with clearance rate of vitamin C at 1 mg/mL.

关 键 词： 黄酮 苦瓜 超临界 提取

领　　域： [轻工技术与工程] [轻工技术与工程]