作　　者： ; ; ; ; ; ; ;

机构地区： 中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室

出　　处： 《水产学报》 2010年第1期132-138,共7页

摘　　要： 海藻食品中无机砷的含量是水产品质量检验中主要的安全卫生指标。实验采用高效液相色谱氢化物发生原子荧光联用技术(HPLC-HG-AFS)对海藻食品中无机砷的检测进行了研究。通过实验优化了提取剂、提取时间及其仪器条件,建立了HPLC-HG-AFS联用技术检测海藻食品中无机砷含量的方法。用1.2mol/LHCl在70℃水浴下提取1h,用过氧化氢(H2O2)氧化;pH6.0的15mmol/L(NH4)2HPO4溶液为流动相,HPLC-HG-AFS上机分析。样品加标量在0.10mg/kg和1.00mg/kg时的平均回收率均在92%以上,相对标准偏差均低于4%,方法精密度较高。研究结果表明,该方法测定海藻中的无机砷较为准确、可靠,为制定农业行业标准海藻食品中无机砷的测定,提供技术支持。 The inorganic arsenic （iAs） in seaweed food is an important safety index in seafood quality detection. High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry （HPLC-HG-AFS） was used to determine the iAs in seaweed food. The concentration of extraction reagents, the extraction time and instrument conditions were optimized. A new method to determine the content of inorganic arsenic in seaweed food using HPLC-HG-AFS was obtained： firstly the seaweeds were extracted with 1.2 mol/L HCl at 70 ℃ for 1 h , then the extracts were oxidized with hydrogen peroxide, and finally were analyzed by HPLC-HG-AFS under the condition of 15 mmot/L（ NH4 ）2HPO4 flow phase （ pH = 6.0）. The average recovery rates of 0.10 mg/kg and 1.00 mg/kg As Ⅲ in samples were all above 92% and the relative standard deviations were all below 4% with a high accuracy. The present study proved that HPLC-HG-AFS was a reliable method to determine the iAs in seaweed food and provide enough evidences for the establishment of the standard for iAs in seaweed food.

关 键 词： 高效液相色谱 氢化物发生原子荧光 海藻 无机砷

领　　域： [生物学] [环境科学与工程]