作　　者： ; ; ; ; ; ;

机构地区： 河北北方学院

出　　处： 《河北北方学院学报（自然科学版）》 2009年第6期21-23,28,共4页

摘　　要： HPLC法测定金莲花中荭草苷和牡荆苷的含量.采用高效液相色谱法（HPLC）,对金莲花中荭草苷和牡荆苷进行含量测定.色谱条件：色谱柱为Hypersil BDS C18;流动相为乙腈—1%醋酸溶液（15∶85）;流速为1.0 mL.min^-1;柱温为25℃;检测波长为340 nm.荭草苷在13.542433.333μg.mL^-1呈良好的线性关系（r=0.999 9）平均回收率为101.32%,RSD=2.4%;牡荆苷在8.854283.333μg.mL^-1呈良好的线性关系（r=0.999 7）平均回收率为100.32%,RSD=1.4%.荭草苷的理论塔板数为5 998,牡荆苷的理论塔板数7 287.方法可靠.用丙酮提取色素后的金莲花残渣中荭草苷和牡荆苷的含量与金莲花原药中的荭草苷和牡荆苷含量相比稍有变化. To determine the contents of orientin and vitexin in Trollius chinesis.With HPLC,the separation was carried out on Hypersil BDS C18 column with a mixture of acetonitrile-1% acetic acid solution（15∶85）as the mobile phase,at a flow rate of 1.0 mL·min^-1.The column temperature was at 25℃.The detection wavelength was at 340nm.Orientin showed a good linear relationship in the range of 13.542433.333μg·mL^-1（r=0.9999）,and the average recovery was 101.32%,RSD=2.4%.Vitexin showed a good linear relationship in the range of 8.854283.333μg·mL^-1（r = 0.9997） and the average recovery was 100.32%,RSD=1.4%.The theoretical plate number of orientin was not less than 5000;Vitexin theoretical plate number was no less than 5900.The method is sensitive and reliable.Compared with the original Trollius chinesis,the contents of orientin and vitexin in acetone extraction of Trollius chinesis are no significant

关 键 词： 金莲花 荭草苷 牡荆苷

领　　域： [理学] [理学]