作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院生物工程学系

出　　处： 《中国生物化学与分子生物学报》 2009年第12期1143-1148,共6页

摘　　要： pUL23是人巨细胞病毒(HCMV)UL23基因编码的皮层蛋白.HCMV皮层蛋白与病毒颗粒的形成、病毒转移、免疫调控等病毒生活过程相关.利用GAL4酵母双杂交系统筛选人胚肾cDNA文库,获得与人巨细胞病毒皮层蛋白pUL23相互作用的宿主蛋白分子DNAJB6[DnaJ(Hsp40)homolog,subfamily B,member 6].回复酵母双杂交、体外GST-Pull down和免疫共沉淀试验再次确认两者之间的相互作用.该结果为进一步研究pUL23蛋白在HCMV生活周期中的作用机制提供依据. Human cytomegalovirus（HCMV） is an opportunistic human pathogen that can cause life-threatening diseases in immunologically immature or compromised individuals,including neonates,AIDS patients and transplant recipients.UL23 gene encodes a tegument protein（pUL23） about 33 kD,of which the role has been implicated in the formation,transport,immunomodulation and transactivation of HCMV.To investigate the underlying function of UL23 during HCMV infections,we screened a human fetal kidney cDNA library using the GAL4 yeast two-hybrid assay.One of the positive clones was identified as the DNAJB6 [DnaJ（Hsp40） homolog,subfamily B,member 6].The interaction between DNAJB6 and pUL23 was further confirmed by GST-pull down and co-immunoprecipitation（Co-IP） experiment with cos-7 cells co-transfected with two plasmids containing DNAJB6 and pUL23.These results may provide important information to the understanding of pUL23 in the HCMV life cycle.

关 键 词： 皮层蛋白 人巨细胞病毒 酵母双杂交 蛋白质相互作用

领　　域： [生物学] [生物学]