作　　者： ; ;

机构地区： 汕头大学医学院

出　　处： 《生理学报》 1998年第4期474-478,共5页

摘　　要： 介绍一种用胶原酶分离和初步鉴定大鼠窦房结起搏细胞的方法。操作简单方便，用酶量少。分离的起搏细胞与原位窦房结起搏细胞具有相类似的动作电位特点：有明显舒张期自动去极化，最大舒张电位平均-55mV，动作电位幅度平均58mV，APD50平均18ms，APD90平均29ms。为进一步的电生理学和组织化学研究提供适当标本。 A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action Potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55 mV, mean peak potential value of 58 mV, and mean APD50 of 18 ms, were similar to those of P cells In situ.

关 键 词： 窦房结 细胞 心肌细胞 胶原酶 大鼠

领　　域： [生物学]