作　　者： ; ; ; ; ;

机构地区： 宁波大学信息科学与工程学院宁波市新型功能材料及其制备科学国家重点实验室培育基地

出　　处： 《中国粮油学报》 2009年第12期124-128,共5页

摘　　要： 在印刷电极表面涂覆一层壳聚糖（Chit）膜,在膜上同时固定四羧基酞菁铁（Ⅲ）（FePc）和HRP酶标黄曲霉毒素B1抗体（HRP-Ab-AFB1）包被纳米金,制备了可用于小麦粉中黄曲霉素B1（AFB1）快速检测的新型安培免疫传感器（SPCE｜Chit/FePc/Au/HRP-Ab-AFB1）。FePc对H2O2的还原具有催化作用,可作为HRP酶与电极之间电子传递媒介体。当该传感器在含AFB1样品的30℃溶液中温育8 min后,AFB1与Ab-AFB1的免疫结合导致HRP的活性中心与FePc之间的电子传递被部分阻碍,使HRP对H2O2电催化氧化电流Io降低。ΔIo与AFB1浓度在1.0-200μg/L成线性关系,标准样品的组内和组间变异系数〈3.5%;回收率97%-104%,检测限为0.3μg/L。该传感器检测AFB1温育时间短,一步测定免分离,为小麦粉中的AFB1现场分析提供了新技术。 A novel amperometric immunosensor for rapid determination of aflatoxin B1（AFB1） in wheat flour based on AFB1 antibody coated nano gold particles modified electrode is proposed.The immunosensor is prepared by co-immobilizing HRP labeled monoclonal antibody of AFB1（HRP-AFB1）,Fe（Ⅲ） Phthlocyanine on the surface of a nano Au collide-modified SPCE electrode by Christon membrane（SPCE｜ Chit/FePc/Au/HRP-Ab-AFB1）.FePc can catalyze the reduction of H2O2 and act as electron mediator between the electrode and HRP during determination. After the immunosensor is incubated with AFB1 solution at 30℃ for 8 min, the access of activity center of the HRP to electrode is partly inhibited, which leads to a linear decrease of catalytic efficiency of the HRP to the oxidation of immobilized thionine by H2O2 in AFB1 concentration at 1.0 to 200 μg/L and the detection limit is 0. 3 μg/L. Being successfully utilized for determination of AFB1 antigen in extraction samples of wheat flour, the immunosensor shows a better accuracy and more rapidly. This method would be valuable for screen determination of AFB1 in flours.

关 键 词： 四羧基酞菁铁 纳米 黄曲霉毒素 安培免疫传感器 小麦粉

领　　域： [理学] [理学]