作　　者： ; ; ; ;

机构地区： 华南理工大学生物科学与工程学院

出　　处： 《西北植物学报》 2009年第11期2153-2161,共9页

摘　　要： 以可直接饲用的马铃薯作为伪狂犬病病毒糖蛋白基因gD的表达植物,探索用马铃薯茎段作为外植体的再生体系和遗传转化体系。结果表明：添加2 mg/L ZT和0.1 mg/L NAA的MS培养基,可使马铃薯茎段愈伤组织诱导率达70%,出芽率达38%;添加0.5 mg/L的硝酸银可明显减少愈伤组织在继代过程中的褐化程度,并且促进其分化。在农杆菌的菌液OD600值为0.1-0.2,侵染茎段1 h,共培养3 d的条件下转化效果最好,抗性愈伤率达到52.3%。实验共获得21株转化植株,挑选7株进行RT-PCR检测,结果证明gD基因已被整合到马铃薯基因组中。 In the research we used potato as bioreactor to express Glycoprotein D gene（gD） from the pseudorabies virus and studied its regeneration system and genetic transformation system with potato stem as the explant.The results indicated that adding 2 mg/L ZT and 0.1 mg/L NAA to the MS medium can increase the rate of callus induction to 70% and the rate of shoot regeneration to 38%.Adding 0.5 mg/L AgNO3 can prevent callus from browning and promote callus regeneration.The optimal transformation system was established and the rate of resistant callus can reach 52.3% in the condition of 0.10.2 OD600 of the Agrobacterium,1 h of infection time and 3 days of co-culture time.Putative 21 transgenic lines were obtained and seven of them were selected for RT-PCR detection.The result confirmed that gD gene had been integrated into potato genomes.

关 键 词： 马铃薯 伪狂犬病病毒 基因 根癌农杆菌介导 遗传转化

领　　域： [生物学]