机构地区: 安徽师范大学生命科学学院生物系
出 处: 《安徽师大学报》 1998年第3期232-235,共4页
摘 要: 卵巢用Carnoy固定,石蜡包埋、切片、用HE染色,PAS反应和RNA反应,光镜观察显示:中华鳖卵细胞可分为卵原细胞期,初级卵泡期,生长卵泡期和成熟卵泡期.PAS反应显示卵透明带、卵黄颗粒呈强阳性,RNA反应显示颗粒层、核仁呈强阳性. The ovary was fixed in Carnoy's fluid and sectioned in paraffin. The staining methed was haematoxylin and eosin. Histochemical techniques adopted RNA and PAS reaction. The results shows: The eggs of Pelodiscus sinensis classities the oogonia, the primary follicale, the growing follicle and the mature eggs. In PAS reaction the zone of pellacida and the yolk granules shows strongly positive. In RNA reaction the granulosa and the nucleolus shows strongly positive.