作　　者： ; ; ; ;

机构地区： 武汉大学人民医院

出　　处： 《现代检验医学杂志》 2009年第6期24-26,共3页

摘　　要： 目的结合PCR与反向膜杂交技术，探讨建立快速筛查细菌中质粒介导的喹诺酮耐药基因qnr的方法。方法将qnr各亚型的特异性探针加尾后固定于尼龙膜上，用标记生物素的各亚型引物分别扩增经提取的细菌DNA，与固定在膜上的探针杂交。结果25株qnr阳性菌株中，有6株qnrA阳性菌株，9株qnrB阳性菌株，10株qnrS阳性菌株。所有阳性菌株均能通过反向膜杂交检测到相应的qnr基因的亚型，且可以在同一张膜上进行检测。结论该方法能够快速、准确地检测出临床病原微生物中质粒介导的喹诺酮类耐药基因qnr，这对于指导临床及时合理用药，采取措施防止耐药菌的传播均有重要意义。 Objective To establish a method for rapid screening qnr genes by PCR and reverse membrane hybridization. Methods Oligonucleotide probes for the subtype of qnr gene were added homopoly talls and then inoculated on hybond membrane. The DNA from the organisms were amplified with biotin labeled universal primers. The PCR products were detected and identified by hybridization with the probes on the membrane. Results All the 25 qnr-positive strains includied 6 qnrA-positive strains, 9 qnr B-positive strains and 10 qnrS-positive strains. And all the subtype of qnr gene could be detected rapidly by reverse membrane hybridization. Conclution By the method of established reverse membrane hybridization, can rapidly screen the qnr genes in gram negative bacteria isolates. It was important to guide clinical drug reasonably,rapidly and prevent the spread of resistant bacteria.

关 键 词： 反向膜杂交 基因 快速检测

领　　域： [生物学]