作　　者： ; ; ; ; ;

机构地区： 鲁东大学生命科学学院

出　　处： 《食品科学》 2009年第21期221-224,共4页

摘　　要： 采用盐析和离子交换层析法制备蛹虫草纤溶酶粗酶样品,并对其酶学性质进行初步研究。结果显示:该酶最适pH值和作用温度分别为8.0和37℃;在pH6～10.6范围内较稳定,但对高温较为敏感;EDTA对蛹虫草纤溶酶具有抑制作用,说明该酶是一种基质金属蛋白酶;金属离子Ca2+、Fe2+在低浓度(0.01mmol/L)时对此酶有促进作用,但高浓度时有抑制作用,而NH4+对酶活性影响不大。 Fibrinolytic enzyme from Cordyceps militaris was extracted by ammonium sulfate precipitation and ion exchange chromatography and its enzymological properties wee also investigated. Results indicated that optimal pH and reaction temperature of this fibrinolytic enzyme were 8.0 and 37 ℃, respectively. This enzyme was stable at pH from 6.0 to 10.6, but sensitive to high temperature. Its activity could be inhibited by EDTA, which suggested this enzyme was a metalloproteinase. Moreover, its activity could be inhibited by EDTA, which suggested this enzyme was a metalloproteinase. Moreover, its activity could be enhanced by Fe^2＋ and Ca^2＋ at a low concentration of 0.01 mol/L and inhibited by them at a high concentration of 0.1 mol/L. However, Na^＋ and NH4^＋had little effect on the activity of this enzyme.

关 键 词： 蛹虫草 发酵 纤溶酶 酶学性质

领　　域： [生物学]